Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

DEVARASU, Thirunavukkarasu, SAAD, Roxane, OUADI, Ali, FRISCH, Benoit, ROBINET, Eric, LAQUERRIERE, Patrice, VOEGEL, Jean Claude, BAUMERT, Thomas, OGIER, Joelle et MEYER, Florent, 2013. Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors. Journal of Materials Chemistry B [en ligne]. 8 juillet 2013. Vol. 1, n° 36pp. 4692-4700. [Consultésans date]. DOI 10.1039/c3tb20557f. Consulté de : http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb20557f#!divAbstract1. .
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Version finale publiée (post-print éditeur)
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Auteurs Thirunavukkarasu Devarasu
Roxane Saad
Ali Ouadi
Benoit Frisch
Eric Robinet
Patrice Laquerriere
Jean Claude Voegel
Thomas Baumert
Joelle Ogier
Florent Meyer
Unité de recherche du site Biomatériaux et bioingénierie - BB - UMRS1121
Institut Pluridisciplinaire Hubert Curien - IPHC - UMR7178
Conception et Application de Molécules Bioactives - CAMB - UMR7199
Institut de recherche sur les maladies virales et hépatiques - IVH - UMRS1110
URL éditeur http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb20557f#!divAbstract
Langue

en
Volume

1
Numéro

36
Page de début

4692
Page de fin

4700
Date de première publication

2013-07-08
Date de parution

213
ISSN

2050-750X
Titre de la source (revue, livre…)

Journal of Materials Chemistry B
Résumé

The present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These “easy to produce” nanoparticles
Show moreThe present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These “easy to produce” nanoparticles (NPs) present an efficient gene silencing effect demonstrated in vitro in a luciferase expressing cell culture model and in vivo in a tumour xenograft mouse model. The luciferase gene silencing percentage reached up to 95% in vitro with biocompatible doses of siRNA. Interestingly, we show by SPECT imaging of radiolabeled particles that without modifying the size, stability and in vitro efficiency, the grafting of a sugar moiety on PEI can modify the in vivo biodistribution of the particles. The proof of concept that galactose-grafting on PEI could change biodistribution without changing the gene silencing efficiency makes them versatile tools for specific delivery of small interfering RNA. As they have been designed so far, biodistribution is mainly located in the liver and thus these innovative nanoparticles open a realistic and feasible strategy for siRNA delivery into the liver in vivo.
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DOI 10.1039/c3tb20557f
Éditeur

Royal Society of Chemistry
Type de publication

journal article
Type de publication

ACL
Domaine

Aucun
Fonction

aut
Audience

International