Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

Devarasu, Thirunavukkarasu; Saad, Roxane; Ouadi, Ali; Frisch, Benoit; Robinet, Eric; Laquerriere, Patrice; Voegel, Jean Claude; Baumert, Thomas; Ogier, Joelle; Meyer, Florent

doi:10.1039/c3tb20557f

Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors

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DEVARASU, Thirunavukkarasu, SAAD, Roxane, OUADI, Ali, FRISCH, Benoit, ROBINET, Eric, LAQUERRIERE, Patrice, VOEGEL, Jean Claude, BAUMERT, Thomas, OGIER, Joelle et MEYER, Florent, 2013. Potent calcium phosphate nanoparticle surface coating for in vitro and in vivo siRNA delivery: a step toward multifunctional nanovectors. Journal of Materials Chemistry B [en ligne]. 8 juillet 2013. Vol. 1, n° 36pp. 4692-4700. [Consultésans date]. DOI 10.1039/c3tb20557f. Consulté de : http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb20557f#!divAbstract1. .

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Auteurs	Thirunavukkarasu Devarasu Roxane Saad Ali Ouadi Benoit Frisch Eric Robinet Patrice Laquerriere Jean Claude Voegel Thomas Baumert Joelle Ogier Florent Meyer
Unité de recherche du site	Biomatériaux et bioingénierie - BB - UMRS1121 Institut Pluridisciplinaire Hubert Curien - IPHC - UMR7178 Conception et Application de Molécules Bioactives - CAMB - UMR7199 Institut de recherche sur les maladies virales et hépatiques - IVH - UMRS1110
Langue	en
Volume	1
Numéro	36
Page de début	4692
Page de fin	4700
Date de première publication	2013-07-08
Date de parution	213
ISSN	2050-750X
Titre de la source (revue, livre…)	Journal of Materials Chemistry B
Résumé	The present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These “easy to produce” nanoparticles Show moreThe present study describes hybrid nanoparticles, built by alternate deposition of siRNA and modified polyethyleneimine (tyrosine-grafted PEI or tyrosine/galactose-grafted PEI) on calcium phosphate nanoparticles. These “easy to produce” nanoparticles (NPs) present an efficient gene silencing effect demonstrated in vitro in a luciferase expressing cell culture model and in vivo in a tumour xenograft mouse model. The luciferase gene silencing percentage reached up to 95% in vitro with biocompatible doses of siRNA. Interestingly, we show by SPECT imaging of radiolabeled particles that without modifying the size, stability and in vitro efficiency, the grafting of a sugar moiety on PEI can modify the in vivo biodistribution of the particles. The proof of concept that galactose-grafting on PEI could change biodistribution without changing the gene silencing efficiency makes them versatile tools for specific delivery of small interfering RNA. As they have been designed so far, biodistribution is mainly located in the liver and thus these innovative nanoparticles open a realistic and feasible strategy for siRNA delivery into the liver in vivo. Show less
DOI	10.1039/c3tb20557f
Éditeur	Royal Society of Chemistry
URL éditeur	http://pubs.rsc.org/en/Content/ArticleLanding/2013/TB/c3tb20557f#!divAbstract
Type de publication	journal article
Type de publication	ACL
Domaine	Aucun
Fonction	aut
Identifiant idREF	225833751 061388505 059307897 138783993 116012714 143387898 075540177
Audience	International
URL	https://univoak.eu/islandora/object/islandora:52619