Annexin A2-dependent actin bundling promotes secretory granule docking to the plasma membrane and exocytosis.
| Accès libre Peer reviewed | |
|---|---|
| Version finale publiée (post-print éditeur) | |
| Paternité - Pas d'utilisation commerciale - Pas de modification [CC] [BY] [NC] [ND] | |
| Auteurs |
Marion Gabel Franck Delavoie Valerie Demais Cathy Royer Yannick Bailly Nicolas Vitale Marie-France Bader Sylvette Chasserot |
| Unité de recherche du site |
Institut des Neurosciences Cellulaires et Intégratives - INCI - UPR3212 Neuropsychologie cognitive et physiopathologie de la schizophrénie - NCPS - UMRS1114 |
| Langue |
en |
| Volume |
210 |
| Numéro |
5 |
| Page de début |
785 |
| Page de fin |
800 |
| Date de première publication |
2015-08-31 |
| Date de parution |
2015-08-31 |
| Titre de la source (revue, livre…) |
The Journal of cell biology |
| Résumé |
Annexin A2, a calcium-, actin-, and lipid-binding protein involved in exocytosis, mediates the formation of lipid microdomains required for the structural and spatial organization of fusion sites at the plasma membrane. To understand how annexin A2 Show moreAnnexin A2, a calcium-, actin-, and lipid-binding protein involved in exocytosis, mediates the formation of lipid microdomains required for the structural and spatial organization of fusion sites at the plasma membrane. To understand how annexin A2 promotes this membrane remodeling, the involvement of cortical actin filaments in lipid domain organization was investigated. 3D electron tomography showed that cortical actin bundled by annexin A2 connected docked secretory granules to the plasma membrane and contributed to the formation of GM1-enriched lipid microdomains at the exocytotic sites in chromaffin cells. When an annexin A2 mutant with impaired actin filament-bundling activity was expressed, the formation of plasma membrane lipid microdomains and the number of exocytotic events were decreased and the fusion kinetics were slower, whereas the pharmacological activation of the intrinsic actin-bundling activity of endogenous annexin A2 had the opposite effects. Thus, annexin A2-induced actin bundling is apparently essential for generating active exocytotic sites. Show less |
| DOI | 10.1083/jcb.201412030 |
| Éditeur |
Rockefeller University Press |
| URL éditeur |
http://jcb.rupress.org/content/210/5/785.long |
| Titre abrégé de la source |
J Cell Biol |
| Type de publication |
journal article |
| Type de publication |
ACL |
| Topic |
Sciences du Vivant [q-bio]/Neurosciences [q-bio.NC] |
| PMID | 26323692 |
| PMCID | PMC4555831 |
| Équipes de recherche |
E1 - Trafic membranaire dans les cellules du système nerveux |
| Fonction |
aut |
| Identifiant idREF |
203755588 185476376 142619957 136946283 |
| Audience |
Non spécifiée |
| Envoyer vers HAL |
5 |
| URL | https://univoak.eu/islandora/object/islandora:63913 |