The 50 distal amino acids of the 2AHP homing protein of Grapevine fanleaf virus elicit a hypersensitive reaction on Nicotiana occidentalis

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MARTIN, Isabelle R., VIGNE, Emmanuelle, BERTHOLD, François, KOMAR, Veronique, LEMAIRE, Olivier, FUCHS, Marc et SCHMITT-KEICHINGER, Corinne, 2018. The 50 distal amino acids of the 2AHP homing protein of Grapevine fanleaf virus elicit a hypersensitive reaction on Nicotiana occidentalis. Molecular Plant Pathology [en ligne]. 2018. Vol. 19, n° 3pp. 731-743. [Consultésans date]. DOI 10.1111/mpp.12558. Consulté de : https://doi.org/10.1111/mpp.125581. .
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Auteurs Isabelle Martin
Emmanuelle Vigne
François Berthold
Veronique Komar
Olivier Lemaire
Marc Fuchs
Corinne Schmitt-Keichinger
Unité de recherche du site Santé de la Vigne et Qualité du Vin - SVQV - UMRA1131
Institut de Biologie Moléculaire des Plantes - IBMP - UPR2357
Langue

en
Volume

19
Numéro

3
Page de début

731
Page de fin

743
Date de première publication

2018
ISSN

1464-6722
Titre de la source (revue, livre…)

Molecular Plant Pathology
Résumé

Summary Avirulence factors are critical for the arm's race between a virus and its host in determining incompatible reactions. The response of plants to viruses from the genus Nepovirus in the family Secoviridae, including Grapevine fanleaf virus
Show moreSummary Avirulence factors are critical for the arm's race between a virus and its host in determining incompatible reactions. The response of plants to viruses from the genus Nepovirus in the family Secoviridae, including Grapevine fanleaf virus (GFLV), is well characterized, although the nature and characteristics of the viral avirulence factor remain elusive. By using infectious clones of GFLV strains F13 and GHu in a reverse genetics approach with wild-type, assortant and chimeric viruses, the determinant of necrotic lesions caused by GFLV-F13 on inoculated leaves of Nicotiana occidentalis was mapped to the RNA2-encoded protein 2AHP, particularly to its 50 C-terminal amino acids. The necrotic response showed hallmark characteristics of a genuine hypersensitive reaction, such as the accumulation of phytoalexins, reactive oxygen species, pathogenesis-related protein 1c and hypersensitivity-related (hsr) 203J transcripts. Transient expression of the GFLV-F13 protein 2AHP fused to an enhanced green fluorescent protein (EGFP) tag in N. occidentalis by agroinfiltration was sufficient to elicit a hypersensitive reaction. In addition, the GFLV-F13 avirulence factor, when introduced in GFLV-GHu, which causes a compatible reaction on N. occidentalis, elicited necrosis and partially restricted the virus. This is the first identification of a nepovirus avirulence factor that is responsible for a hypersensitive reaction in both the context of virus infection and transient expression.
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DOI 10.1111/mpp.12558
Éditeur

John Wiley & Sons, Ltd (10.1111)
URL éditeur https://doi.org/10.1111/mpp.12558
Type de publication

journal article
Type de publication

ACL
Domaine

Aucun
Unité de recherche extérieure au site

Department of Plant Pathology and Plant–Microbe Biology, Cornell University, New York State Agricultural Experiment Station, Geneva, NY, 14456, USA
Fonction

aut
Identifiant idREF 238438864
177139684
196936292
033949468
112183379
238439542
Audience

Non spécifiée
Envoyer vers HAL

1
URL https://univoak.eu/islandora/object/islandora:72942