ABCE1: A special factor that orchestrates translation at the crossroad between recycling and initiation

en

14

10

1279

1285

2017

RNA Biol.

For many years initiation and termination of mRNA translation have been studied separately. However, a direct link between these 2 isolated stages has been suggested by the fact that some initiation factors also control termination and can even Show moreFor many years initiation and termination of mRNA translation have been studied separately. However, a direct link between these 2 isolated stages has been suggested by the fact that some initiation factors also control termination and can even promote ribosome recycling; i.e. the last stage where post-terminating 80S ribosomes are split to start a new round of initiation. Notably, it is now firmly established that, among other factors, ribosomal recycling critically requires the NTPase ABCE1. However, several earlier reports have proposed that ABCE1 also somehow participates in the initiation complex assembly. Based on an extended analysis of our recently published late-stage 48S initiation complex from rabbit, here we provide new mechanistic insights into this putative role of ABCE1 in initiation. This point of view represents the first structural evidence in which the regulatory role of the recycling factor ABCE1 in initiation is discussed and establishes a corner stone for elucidating the interplay between ABCE1 and several initiation factors during the transit from ribosomal recycling to formation of the elongation competent 80S initiation complex. Show less

Mancera-Martinez, Eder Brito Querido, Jailson Valasek, Leos Shivaya Simonetti, Angelita Hashem, Yaser eng Review Research Support, Non-U.S. Gov't 2017/05/13 06:00 RNA Biol. 2017 Oct 3;14(10):1279-1285. doi: 10.1080/15476286.2016.1269993. Epub 2017 May 12.

journal article

ACL

Aucun
Animals
Binding Sites
Hydrolysis
Models, Molecular
Nucleosides/*chemistry
*Peptide Chain Initiation, Translational
Peptide Chain Termination, Translational
Peptide Elongation Factors
Peptide Initiation Factors/metabolism
Protein Binding
Rabbits
Ribosomes/metabolism
*Abce1
*cryo-EM
*recycling
*ribosome
*translation tRNA

ATP-Binding Cassette Transporters/*chemistry/*metabolism
Animals
Binding Sites
Hydrolysis
Models, Molecular
Nucleosides/*chemistry
*Peptide Chain Initiation, Translational
Peptide Chain Termination, Translational
Peptide Elongation Factors
Peptide Initiation Factors/metabolism
Protein Binding
Rabbits
Ribosomes/metabolism
*Abce1
*cryo-EM
*recycling
*ribosome
*translation tRNA
tRNA

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