Association of porous hydroxyapatite and bone marrow cells for bone regeneration

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ANSELME, Karine, NOËL, B., FLAUTRE, B., BLARY, M.-C, DELECOURT, C., DESCAMPS, M. et HARDOUIN, P., sans date. Association of porous hydroxyapatite and bone marrow cells for bone regeneration. Bone. sans date. Vol. 25, n° 2pp. 51S-54S. DOI 10.1016/S8756-3282(99)00133-7. 1. .
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Auteurs Karine Anselme
B Noël
B Flautre
M.-C Blary
C Delecourt
M Descamps
P Hardouin
Langue

en
Volume

25
Numéro

2
Page de début

51S
Page de fin

54S
Date de première publication

1999-08
ISSN

8756-3282
Titre de la source (revue, livre…)

Bone
Résumé

The preparation of hybrid material with osteoinductive capacity may be achieved by association of cultured autologous bone cells with a porous ceramic vehicle. We optimized culture conditions for rabbit marrow stromal stem cells (MSCs), notably by Show moreThe preparation of hybrid material with osteoinductive capacity may be achieved by association of cultured autologous bone cells with a porous ceramic vehicle. We optimized culture conditions for rabbit marrow stromal stem cells (MSCs), notably by selection from batches of fetal calf serum. Rabbit MSCs formed colony-forming unit-fibroblastic (CFU-Fs) in vitro. Their alkaline phosphatase (ALP) activity was doubled in the presence of dexamethasone. Autologous rabbit serum allowed the formation of ALP-positive CFU-Fs, but results were highly variable depending on the rabbit. We tested the osteogenic potential of autologous cultured (with or without dexamethasone addition in the culture medium) and noncultured rabbit MSCs associated with a porous hydroxyapatite ceramic after a dorsal intramuscular implantation. Nucleated cells (107 or 108/mL) were used for the preparation of autologous hybrid material. A significantly higher number of implants containing bone was obtained with a suspension of 107 cells/mL cultured in the presence of 10−8 M dexamethasone. Some positive implants were also obtained with a suspension of 108 noncultured cells/mL. We demonstrated the feasibility of preparing rabbit autologous hybrid materials following a process for controlling culture conditions, cell characterization and cell/material association. Show less
DOI 10.1016/S8756-3282(99)00133-7
Éditeur

Elsevier
Titre abrégé de la source

Bone
Type de publication

ACL
Domaine

Sciences du Vivant [q-bio]/Ingénierie biomédicale
Mots-clés

marrow stromal cells
rabbit
in vitro differentiation
ceramic
hybrid material
osteogenic potential
Unité de recherche extérieure au site

Institut de Recherche sur les Biomatériaux et les Biotechnologies (IR2B), Université du Littoral Côte d’Opale, Berck sur Mer, France
Fonction

aut
Identifiant idREF 033446792
Audience

Non spécifiée
Envoyer vers HAL

5
URL https://univoak.eu/islandora/object/islandora:92023